Echuca, Bendigo and Swan Hill passengers will be impacted
There will be more cancellations on the Bendigo, Swan Hill and Echuca rail lines on February 7th.
Passengers will be forced to use replacement buses due to further industrial action.
This is the second round of 24-hour V-Line strikes in the pay dispute between the Andrew's Government and the state's transport union.
Shadow Minister for Regional Public Transport Steph Ryan said passengers should not have to be caught in the crossfire.
"The VLine network is a critical link between country communities and Melbourne," she said.
"Every day these strikes force the cancellation of trains is another day that country people can't go about their business.
"It's time embattled Public Transport Minister Melissa Horne steps up and resolves this mess."
The first strike on the same lines occurred in December, and the leader of the Nationals and member for Murray Plains, Peter Walsh, said it is an inconvenience to locals.
"For the second time in as many months, Swan Hill and Bendigo line passengers will face more delays and cancellations due to Daniel Andrews’ industrial chaos," he said.
"Every day of disruption is another day country Victorians will find it harder to get where we need to be, whether it’s for work, medical appointments or to see family."